1、 Preparation stage
Material selection: Choose fresh, impurity free, and high-quality mushrooms as raw materials. The types of mushrooms can be selected according to needs, such as Agaricus bisporus, Mongolian mushroom, etc.
Pre treatment: Drying, crushing and other pre-treatment steps are carried out on mushrooms to reduce particle size, increase the contact area between solvent and mushrooms, and improve extraction efficiency. The crushed mushroom powder usually needs to be sieved through a sieve with a certain mesh size to ensure the uniformity of particle size.
2、 Ultrasonic extraction
Equipment selection: Choose suitable ultrasonic extraction equipment, such as ultrasonic cleaner, ultrasonic extractor, etc. These devices are capable of generating high-frequency ultrasonic vibrations and transmitting them into mushroom powder.
Solvent selection: Choose the appropriate solvent based on the properties of the target component. Common solvents include water, ethanol, methanol, etc. The selection of solvents should take into account factors such as the solubility of the target component, the toxicity of the solvent, and cost.
Extraction operation: Mix the pre treated mushroom powder with a solvent in a certain proportion and place it in an ultrasonic extraction device for extraction. During the extraction process, ultrasonic vibration can damage the cell wall and membrane of mushrooms, causing the target components to be rapidly released and dissolved into the solvent. The parameters such as extraction time, temperature, and ultrasonic power can be adjusted as needed to optimize the extraction effect.
3、 Subsequent processing
Centrifugal separation: After extraction, the extract is subjected to centrifugal separation to remove solid impurities and undissolved mushroom powder. The speed and time of centrifugal separation should be adjusted according to the actual situation.
Concentration and purification: Concentrate and purify the extracted liquid after centrifugal separation as needed. Common concentration methods include vacuum distillation, vacuum concentration, etc; Purification methods include extraction, chromatography, crystallization, etc.
Drying and preservation: The purified target component is dried to remove solvents and moisture, resulting in a dried target product. The dried product should be sealed and stored in a cool and dry place to prevent moisture degradation and deterioration.